luxI

BBa_C0061 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_C0061
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr
Date created: 2003-01-31 12:00:00
Date modified: 2015-08-31 04:07:23

autoinducer synthetase for AHL



Types
DnaRegion

Roles
Coding

CDS

Sequences BBa_C0061_sequence (Version 1)

Description

Synthesizes 3OC6HSL, which binds to LuxR.

The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the Lux repressor, LuxR. Two molecules of LuxR protein form a complex with two molecules the signalling compound HSL. This complex binds to a palindromic site on the promoter, increasing the rate of transcription.



Notes

An LVA tail (sequence: AANDENYALVA) was added to increase protein degradation. .



Source

V. fischeri AF170104

Sequence Annotation Location Component / Role(s)
LVA
luxI
Help:Barcodes
BBa_C0061
580,611
1,579
619,643
1,618
tag feature/tag
feature/cds CDS
feature/barcode engineered_tag
engineered_region feature/BioBrick
igem#experience None  
igem#partStatus Released HQ 2013  
igem#sampleStatus In stock  
igem#status Available  
synbiohub#ownedBy user/james  
synbiohub#ownedBy user/myers  
synbiohub#topLevel BBa_C0061/1