luxI

BBa_C0061 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_C0061
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr
Date created: 2003-01-31 12:00:00
Date modified: 2015-08-31 04:07:23

autoinducer synthetase for AHL



    • Details

    Types
    DnaRegion

    Roles
    Coding

    CDS

    Sequences BBa_C0061_sequence (Version 1)

    Description

    Synthesizes 3OC6HSL, which binds to LuxR.

    The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the Lux repressor, LuxR. Two molecules of LuxR protein form a complex with two molecules the signalling compound HSL. This complex binds to a palindromic site on the promoter, increasing the rate of transcription.



    Notes

    An LVA tail (sequence: AANDENYALVA) was added to increase protein degradation. .



    Source

    V. fischeri AF170104

    Sequence Annotation Location Component / Role(s)
    LVA
    luxI
    Help:Barcodes
    BBa_C0061
    		580,611
    1,579
    619,643
    1,618
    		tag feature/tag
    feature/cds CDS
    feature/barcode engineered_tag
    engineered_region feature/BioBrick

    igem#experience
    None
     
    igem#partStatus
    Released HQ 2013
    igem#sampleStatus
    In stock
    igem#status
    Available
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_C0061/1
     

    Attachments

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