Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr
Date created: 2003-01-31 12:00:00
Date modified: 2015-08-31 04:07:23
luxR repressor/activator, (no LVA?)
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_C0062_sequence (Version 1) |
Description
In complex with HSL, LuxR binds to the Lux promoter, activating transcription from PrThe lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the Lux activator, LuxR complexed to HSL. Two molecules of LuxR protein form a complex with two molecules the signalling compound homoserine lactone (HSL). This complex binds to a palindromic site on the promoter, increasing the rate of transcription.
Notes
2 silent point mutants were introduced in the coding sequence to remove internal XbaI and PstI sites. Mutation sites were chosen to replace codons commonly used in E. coli with codons used at a similar frequency.
Source
V. fischeri| Sequence Annotation | Location | Component / Role(s) |
| prefix T A luxR Help:Barcodes BBa_C0062 | 1,2 174,174 492,492 1,750 757,781 1,756 | engineered_region feature/BioBrick feature/s_mutation point_mutation feature/s_mutation point_mutation feature/cds CDS engineered_tag feature/barcode feature/BioBrick engineered_region |