luxr

BBa_C0062 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_C0062
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr
Date created: 2003-01-31 12:00:00
Date modified: 2015-08-31 04:07:23

luxR repressor/activator, (no LVA?)



Types
DnaRegion

Roles
CDS

Coding

Sequences BBa_C0062_sequence (Version 1)

Description

In complex with HSL, LuxR binds to the Lux promoter, activating transcription from Pr BBa_R0062, and repressing transcription from Pl BBa_R0063.

The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the Lux activator, LuxR complexed to HSL. Two molecules of LuxR protein form a complex with two molecules the signalling compound homoserine lactone (HSL). This complex binds to a palindromic site on the promoter, increasing the rate of transcription.



Notes

2 silent point mutants were introduced in the coding sequence to remove internal XbaI and PstI sites. Mutation sites were chosen to replace codons commonly used in E. coli with codons used at a similar frequency.



Source

V. fischeri AF170104

Sequence Annotation Location Component / Role(s)
prefix
T
A
luxR
Help:Barcodes
BBa_C0062
1,2
174,174
492,492
1,750
757,781
1,756
engineered_region feature/BioBrick
feature/s_mutation point_mutation
feature/s_mutation point_mutation
feature/cds CDS
engineered_tag feature/barcode
feature/BioBrick engineered_region
igem#experience None  
igem#partStatus Released HQ 2013  
igem#sampleStatus In stock  
igem#status Available  
synbiohub#ownedBy user/james  
synbiohub#ownedBy user/myers  
synbiohub#topLevel BBa_C0062/1