Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Stephen Lee, Roshan Kumar, Joe Levine, Ziyan Chu (Polkadorks, IAP 2004)
Date created: 2004-01-27 12:00:00
Date modified: 2015-08-31 04:07:24
Receptor, tar-envZ
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_C0082_sequence (Version 1) |
Description
Coding sequence for Tar-EnvZ fusion protein. Tar is the bacterial chemotaxis receptor for aspartate. EnvZ is an osmosensor kinase, which, through phosphorylation of OmpR, regulates transcription of porin genes.This fusion protein combines the extracellular and transmembrane domains of Tar with the intracellular domain of EnvZ. The fusion converts chemical binding of aspartate to production of transcription factors.
Notes
A silent point mutation was made at base 423 (A -> C) to eliminate an XbaI restriction enzyme site. The mutation was designed to match codons with similar frequencies of usage in E. coli.Source
NC_000913 E. coli K12| Sequence Annotation | Location | Component / Role(s) |
| Help:Barcodes A LVA Tar (front) Envz (back) | 1492,1516 423,424 1453,1485 1,765 772,1452 | feature/barcode engineered_tag point_mutation feature/s_mutation tag feature/tag CDS feature/cds feature/cds CDS |