aspA

BBa_C0083 Version 1

Component

Source: http://parts.igem.org/Part:BBa_C0083

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Stephen Lee, Roshan Kumar, Joe Levine, Ziyan Chu (Polkadorks, IAP 2004)

Date created: 2004-01-27 12:00:00

Date modified: 2015-08-31 04:07:24

aspartate ammonia-lyase

Details

• Types: DnaRegion
• Roles: CDS; Coding
• Sequences: BBa_C0083_sequence (Version 1)

Description

Coding sequence for AspA enzyme. AspA aminates fumarate to make aspartate. Aspartate can be used as a bacterial chemotaxis signal.

Notes

The start codon was changed from GTG to ATG to match BioBricks standards.

Five silent mutations were introduced to eliminate EcoRI and PstI restriction enzyme sites. EcoRI sites were eliminated at bases 588 and 657 with C -> T mutations. PstI sites were eliminated at bases 624, 999, and 1125 with G -> A mutations. The mutations were designed to match codons with similar frequencies of usage in E. coli.

Source

AE000486 E. coli K12

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
Help:Barcodes	1519,1543	feature/barcode, engineered_tag
AspA	1,1479	feature/cds, CDS
G	1,1	point_mutation, feature/s_mutation
C	588,588	feature/s_mutation, point_mutation
G	624,624	feature/s_mutation, point_mutation
C	657,657	feature/s_mutation, point_mutation
G	999,999	feature/s_mutation, point_mutation
G	1125,1125	point_mutation, feature/s_mutation
LVA	1480,1512	tag, feature/tag

Other Properties

• igem#experience: None
• igem#partStatus: Released HQ 2013
• igem#sampleStatus: In stock
• igem#status: Available
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_C0083/1

Member of these Collections

• //cds
• //cds/enzyme
• //function/biosynthesis
• //function/motility
• iGEM Parts Registry