ECFP

BBa_E0022 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_E0022
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: June Rhee, Connie Tao, Ty Thomson, Louis Waldman
Date created: 2003-01-31 12:00:00
Date modified: 2015-08-31 04:07:25

enhanced cyan fluorescent protein derived from A. victoria GFP



    • Details

    Types
    DnaRegion

    Roles
    Reporter

    engineered_region

    Sequences BBa_E0022_sequence (Version 1)

    Description

    Cyan fluorescent protein (ECFP) reporter coding sequence without the Ribosome Binding Site. Modified with an LVA tail for rapid degradation of the protein and faster fall time for the emission.



    Notes

    BBa_E0022 cyan fluorescent protein is based on BioBrick part BBa_E0021. It has been modified to include a rapid degradation LVA tail, and includes the BioBrick standard assembly head and tail restriction sites. The RBS has been removed. The stop codon has been changed from TAA to a double stop codon TAATAA.



    Source

    Modified from BBa_E0021.

    Sequence Annotation Location Component / Role(s)
    CFP (LVA)
    SsrA
    2
    A
    BBa_E0022
    		1,762
    719,756
    757,762
    69,69
    1,762
    		CDS feature/cds
    feature/tag tag
    stop_codon feature/stop
    point_mutation feature/s_mutation
    feature/BioBrick engineered_region

    igem#partStatus
    Released HQ 2013
    igem#sampleStatus
    In stock
    igem#status
    Available
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_E0022/1
     

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