ECFP

BBa_E0022 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_E0022
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: June Rhee, Connie Tao, Ty Thomson, Louis Waldman
Date created: 2003-01-31 12:00:00
Date modified: 2015-08-31 04:07:25

enhanced cyan fluorescent protein derived from A. victoria GFP



Types
DnaRegion

Roles
Reporter

engineered_region

Sequences BBa_E0022_sequence (Version 1)

Description

Cyan fluorescent protein (ECFP) reporter coding sequence without the Ribosome Binding Site. Modified with an LVA tail for rapid degradation of the protein and faster fall time for the emission.



Notes

BBa_E0022 cyan fluorescent protein is based on BioBrick part BBa_E0021. It has been modified to include a rapid degradation LVA tail, and includes the BioBrick standard assembly head and tail restriction sites. The RBS has been removed. The stop codon has been changed from TAA to a double stop codon TAATAA.



Source

Modified from BBa_E0021.

Sequence Annotation Location Component / Role(s)
CFP (LVA)
SsrA
2
A
BBa_E0022
1,762
719,756
757,762
69,69
1,762
CDS feature/cds
feature/tag tag
stop_codon feature/stop
point_mutation feature/s_mutation
feature/BioBrick engineered_region
igem#partStatus Released HQ 2013  
igem#sampleStatus In stock  
igem#status Available  
synbiohub#ownedBy user/james  
synbiohub#ownedBy user/myers  
synbiohub#topLevel BBa_E0022/1