YFP

BBa_E0032 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_E0032
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: June Rhee, Connie Tao, Ty Thomson, Louis Waldman
Date created: 2003-01-31 12:00:00
Date modified: 2015-08-31 04:07:25

enhanced yellow fluorescent protein derived from A. victoria GFP



Types
DnaRegion

Roles
Reporter

engineered_region

Sequences BBa_E0032_sequence (Version 1)

Description

Yellow fluorescent protein (EYFP) reporter coding sequence without the Ribosome Binding Site. Modified with an LVA tail for rapid degradation of the protein and faster fall time for the emission.

Annotated mutation cause a Q81L mutation that appears to not be near the active site.



Notes

BBa_E0032 yellow fluorescent protein is based on BioBrick part BBa_E0031. It has been modified to include a rapid degradation LVA tail, and includes the BioBrick standard assembly head and tail restriction sites. The RBS has been removed. The stop codon has been changed from TAA to a double stop codon TAATAA.



Source

Modified from BBa_E0031

Sequence Annotation Location Component / Role(s)
YFP (LVA)
2
SsrA
A (Q->L)
BBa_E0032
1,762
757,762
718,756
242,242
1,762
CDS feature/cds
feature/stop stop_codon
tag feature/tag
feature/mutation sequence_alteration
engineered_region feature/BioBrick
igem#partStatus Released HQ 2013  
igem#sampleStatus In stock  
igem#status Available  
synbiohub#ownedBy user/james  
synbiohub#ownedBy user/myers  
synbiohub#topLevel BBa_E0032/1