YFP

BBa_E0032 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_E0032
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: June Rhee, Connie Tao, Ty Thomson, Louis Waldman
Date created: 2003-01-31 12:00:00
Date modified: 2015-08-31 04:07:25

enhanced yellow fluorescent protein derived from A. victoria GFP



    • Details

    Types
    DnaRegion

    Roles
    Reporter

    engineered_region

    Sequences BBa_E0032_sequence (Version 1)

    Description

    Yellow fluorescent protein (EYFP) reporter coding sequence without the Ribosome Binding Site. Modified with an LVA tail for rapid degradation of the protein and faster fall time for the emission.

    Annotated mutation cause a Q81L mutation that appears to not be near the active site.



    Notes

    BBa_E0032 yellow fluorescent protein is based on BioBrick part BBa_E0031. It has been modified to include a rapid degradation LVA tail, and includes the BioBrick standard assembly head and tail restriction sites. The RBS has been removed. The stop codon has been changed from TAA to a double stop codon TAATAA.



    Source

    Modified from BBa_E0031

    Sequence Annotation Location Component / Role(s)
    YFP (LVA)
    2
    SsrA
    A (Q->L)
    BBa_E0032
    		1,762
    757,762
    718,756
    242,242
    1,762
    		CDS feature/cds
    feature/stop stop_codon
    tag feature/tag
    feature/mutation sequence_alteration
    engineered_region feature/BioBrick

    igem#partStatus
    Released HQ 2013
    igem#sampleStatus
    In stock
    igem#status
    Available
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_E0032/1
     

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