BBa_E0041

BBa_E0041 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_E0041
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Heather Keller
Date created: 2007-03-05 12:00:00
Date modified: 2015-08-31 04:07:25

SapI + GFP



    • Details

    Types
    DnaRegion

    Roles
    engineered_region

    Reporter

    Sequences BBa_E0041_sequence (Version 1)

    Description

    GFP (mut3) part number E0040 with a unpstream SapI site. Cleave with SapI results in an overhang of 3'-TAC - 5' from the left end of the GFP coding region, making the coding sequence compatible with Heather Keller's alternative assembly method for blunt cloning of RBS sequences upstream of a coding sequence.

    Notes

    n/a

    Source

    The part was generated by PCR amplification of part E0040 with the SapI site incorported into the forward promoter.

    Sequence Annotation Location Component / Role(s)
    SapI recognition
    BBa_B0102
    BBa_E0040
    		1,7
    1,8
    9,728
    		feature/misc sequence_feature
    feature/BioBrick engineered_region
    feature/BioBrick engineered_region

    igem#sampleStatus
    Not in stock
    igem#status
    Unavailable
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#topLevel
    BBa_E0041/1
     

    Attachments

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