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BBa_E0042

BBa_E0042 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_E0042

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Heather Keller

Date created: 2007-03-05 12:00:00

Date modified: 2015-08-31 04:07:25

SapI + mCherry

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Details

Types	DnaRegion
Roles	Reporter engineered_region
Sequences	BBa_E0042_sequence (Version 1)

Description

mCherry part number J06504 with an upstream SapI site. Cleave with SapI results in an overhang of 3'-TAC - 5' from the left end of the mCherry coding region, making the coding sequence compatible with Heather Keller's alternative assembly method for blunt cloning of RBS sequences upstream of a coding sequence.

Notes

n/a

Source

The part was generated by PCR amplification of part E0040 with the SapI site incorported into the forward promoter.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
SapI recognition BBa_B0102 mCherry C->T (removing PstI site) BBa_J06504	1,7 1,8 9,719 360,360 9,722	feature/misc sequence_feature feature/BioBrick engineered_region feature/cds CDS sequence_alteration feature/mutation engineered_region feature/BioBrick