mRFP1

BBa_E1010 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_E1010
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Drew Endy
Date created: 2004-07-27 11:00:00
Date modified: 2015-08-31 04:07:26

**highly** engineered mutant of red fluorescent protein from Discosoma striata (coral)



Types
DnaRegion

Roles
CDS

Coding

Sequences BBa_E1010_sequence (Version 1)

Description

monomeric RFP:
Red Fluorescent Protein.
Excitation peak: 584 nm
Emission peak: 607 nm

Notes

TAATAA double stop codon added (DE).

Four silent mutations made to remove three EcoRI sites and one PstI site: A28G, A76G, A349G, G337A.

Source

Campbell et al., PNAS v99 p7877 URL

Sequence Annotation Location Component / Role(s)
mrfp1
Help:Barcodes
1,675
682,706
feature/cds CDS
engineered_tag feature/barcode
igem#experience Works  
igem#partStatus Released HQ 2013  
igem#sampleStatus In stock  
igem#status Available  
synbiohub#ownedBy user/james  
synbiohub#ownedBy user/myers  
synbiohub#topLevel BBa_E1010/1