mRFP1

BBa_E1010 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_E1010
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Drew Endy
Date created: 2004-07-27 11:00:00
Date modified: 2015-08-31 04:07:26

**highly** engineered mutant of red fluorescent protein from Discosoma striata (coral)



    • Details

    Types
    DnaRegion

    Roles
    CDS

    Coding

    Sequences BBa_E1010_sequence (Version 1)

    Description

    monomeric RFP:
    Red Fluorescent Protein.
    Excitation peak: 584 nm
    Emission peak: 607 nm

    Notes

    TAATAA double stop codon added (DE).

    Four silent mutations made to remove three EcoRI sites and one PstI site: A28G, A76G, A349G, G337A.

    Source

    Campbell et al., PNAS v99 p7877 URL

    Sequence Annotation Location Component / Role(s)
    mrfp1
    Help:Barcodes
    		1,675
    682,706
    		feature/cds CDS
    engineered_tag feature/barcode

    igem#experience
    Works
     
    igem#partStatus
    Released HQ 2013
    igem#sampleStatus
    In stock
    igem#status
    Available
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_E1010/1
     

    Attachments

    © 2018 Newcastle University, University of Utah, and collaborators
    About SynBioHub | View Source on Github | Report an Issue
    | v1.6.1 (86615526)