Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: ryu
Date created: 2005-01-24 12:00:00
Date modified: 2015-08-31 04:07:26
enhanced version of ECFP, yeast-optimized
| Types | DnaRegion |
| Roles | Reporter engineered_region |
| Sequences | BBa_E2020_sequence (Version 1) |
Description
CFP cerulean, yeast codon optimized.Notes
Contains N- and C-terminal linker sequences (with homology to biobrick parts BBa_E2060 (mCherry), BBa_E2050 (mOrange), and BBa_E2030 (Venus YFP) to facilitate color swapping in yeast. Adds N-"MATSG" and "GSGTA"-C. to published amino acid sequence. Inserts a "V" after normal start M. Double TAATAA stop codon. Missing EcoRI, HindIII, NotI, NdeI, XhoI, RsrII, BamHI, NcoI, BglI, SpeI, XbaI, and PstI. Except for 5' and 3' ends no significant sequence identity runs with Venus YFP (BBa_E2030) or to GFP S65T as found in O'Shea deletion strain collection (originally from plasmid pFA6-GFP(S65T)-His3MX6).Source
Rizzo MA, et al. (2004) Nat Biot (22):445-9| Sequence Annotation | Location | Component / Role(s) |
| MATSG linker GSGTA linker Cerulean CFP Help:Barcodes | 1,15 733,747 1,747 754,778 | feature/misc sequence_feature feature/misc sequence_feature feature/cds CDS engineered_tag feature/barcode |