Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: ryu
Date created: 2005-01-24 12:00:00
Date modified: 2015-08-31 04:07:26
enhanced version of EYFP, yeast-optimized
| Types | DnaRegion |
| Roles | engineered_region Reporter |
| Sequences | BBa_E2030_sequence (Version 1) |
Description
Venus YFP, yeast codon optimized. EX515/EM528Notes
Contains N- and C-terminal linker sequences (with homology to biobrick parts BBa_E2060 (mCherry), BBa_E2050 (mOrange), and BBa_E2020 (Cerulean CFP) to facilitate color swapping in yeast. Adds N-"MATSG" and "GSGTA"-C. to published amino acid sequence. Inserts a "V" after normal start M. Double TAATAA stop codon. Missing EcoRI, HindIII, NotI, NdeI, XhoI, RsrII, BamHI, NcoI, BglI, SpeI, XbaI, and PstI. Except for 5' and 3' ends no significant sequence identity runs with Cerulean CFP (BBa_E2020) or to GFP S65T as found in O'Shea deletion strain collection (originally from plasmid pFA6-GFP(S65T)-His3MX6).Source
nagai T. et al. (2002) Nat Biot (20):87-90| Sequence Annotation | Location | Component / Role(s) |
| Venus YFP MATSG linker GSGTA linker Help:Barcodes | 1,747 1,15 733,747 754,778 | CDS feature/cds feature/misc sequence_feature sequence_feature feature/misc feature/barcode engineered_tag |