Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: ryu
Date created: 2005-01-24 12:00:00
Date modified: 2016-01-25 02:35:54
derivative of mRFP1, yeast-optimized
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_E2050_sequence (Version 1) |
Description
mRFP derivative. Ex548nm/Em562. yeast codon optimized.Notes
Contains N- and C-terminal linker sequences (with homology to biobrick parts BBa_E2060 (mCherry), BBa_E2020 (Cerulean CFP), and BBa_E2030 (Venus YFP) to facilitate color swapping in yeast. Adds N-"MATSG" and "GSGTA"-C. to published amino acid sequence. Double TAATAA stop codon. Missing EcoRI, HindIII, NotI, NdeI, XhoI, RsrII, BamHI, NcoI, BglI, SpeI, XbaI, and PstI. except for 5' and 3' ends no significant sequence identity runs to mCherry (BBa_E2060).Source
Shaner et al, 2004. Nat Biotech (22):1567-1571| Sequence Annotation | Location | Component / Role(s) |
| MATSG linker GSGTA linker mOrange Help:Barcodes | 1,15 724,738 1,738 745,769 | sequence_feature feature/misc sequence_feature feature/misc feature/cds CDS engineered_tag feature/barcode |