Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: ryu
Date created: 2005-01-24 12:00:00
Date modified: 2015-08-31 04:07:27
derivative of mRFP1, yeast-optimized
| Types | DnaRegion |
| Roles | Reporter engineered_region |
| Sequences | BBa_E2060_sequence (Version 1) |
Description
mRFP (DsRed) derived, Ex587/Em610, yeast codon optimizedNotes
Codon optimized for yeast. Contains N- and C-terminal linker sequences (with homology to biobrick parts BBa_E2050 (mOrange), BBa_E2020 (Cerulean CFP), and BBa_E2030 (Venus YFP) to facilitate color swapping in yeast. Adds N-"MATSG" and "GSGTA"-C. to published amino acid sequence. Double TAATAA stop codon. Missing EcoRI, HindIII, NotI, NdeI, XhoI, RsrII, BamHI, NcoI, BglI, SpeI, XbaI, and PstI. except for 5' and 3' ends no significant sequence identity runs to mOrange (BBa_E2050).Source
Shaner et al, 2004. Nat Biotech (22):1567-1571| Sequence Annotation | Location | Component / Role(s) |
| MATSG linker GSGTA linker mCherry Help:Barcodes | 1,15 724,738 1,738 745,769 | feature/misc sequence_feature feature/misc sequence_feature CDS feature/cds feature/barcode engineered_tag |