Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: ryu
Date created: 2005-03-11 12:00:00
Date modified: 2015-08-31 04:07:27
FRET acceptor YPet (optimized to work with CyPet (Cyan FP) as donor
| Types | DnaRegion |
| Roles | Reporter engineered_region |
| Sequences | BBa_E3030_sequence (Version 1) |
Description
YPet YFP, optimized CyPet (Cyan FP) FRET acceptorNotes
Contains A206K (in this sequence A211K due to N-terminal linker) mutation to reduce dimerization potential as described by Roger Tsien. Contains N- and C-terminal linker sequences (with homology to biobrick parts BBa_E2060 (mCherry), BBa_E2050 (mOrange), and BBa_E2020 (Cerulean CFP) to facilitate color swapping in yeast. Adds N-"MATSG" and "GSGTA"-C. to published amino acid sequence. Inserts a "V" after normal start M. Double TAATAA stop codon. Missing EcoRI, HindIII, NotI, NdeI, XhoI, RsrII, BamHI, NcoI, BglI, SpeI, XbaI, and PstI. Except for 5' and 3' ends no significant sequence identity runs with above biobrick FPs and GFP S65T as found in O'Shea deletion strain collection (originally from plasmid pFA6-GFP(S65T)-His3MX6).Source
Nguyen et al, (2005) Nature Biotech Feb 6 Epub PMID: 15696158| Sequence Annotation | Location | Component / Role(s) |
| MATSG linker GSGTA linker Help:Barcodes | 1,15 733,747 754,778 | sequence_feature feature/misc feature/misc sequence_feature engineered_tag feature/barcode |