BBa_I1012

BBa_I1012 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_I1012
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: June Rhee, Connie Tao, Ty Thomson, Louis Waldman
Date created: 2003-01-31 12:00:00
Date modified: 2015-08-31 04:07:29

CI(1) micRNA asRNA



Types
DnaRegion

Roles
mature_transcript_region

RNA

Sequences BBa_I1012_sequence (Version 1)

Description

Region which serves as basis for transcription of asRNA that binds to and inhibits BBa_I1010's mRNA transcript via micRNA mechanism (See below for details). Has tetR promoter BBa_R0040. Part of the XOR gate comprised of BBa_I1010 and BBa_I1020, their corresponding asRNA coding sequences (BBa_I1012 and BBa_I1022), and the LacO-1 and TetR BBa_R0040 promoters.

Notes

References (unparsed) here:

Mizuno, T., et al. Proc. Natl. Acad. Sci. USA (1984) 81, 1966-1970.

Coleman, J., et al. Cell (1984) 37, 429-36.

References (unparsed) here:

Mizuno, T., et al. Proc. Natl. Acad. Sci. USA (1984) 81, 1966-1970.

Coleman, J., et al. Cell (1984) 37, 429-36.

Complementary to beginning of BBa_I1010 transcript covering RBS, start codon, and 73 bp into coding sequence. No designed secondary structure. Complementary region is flanked by two stem loop structures, as specified by the micRNA mechanism.

Anti-sense

The success of this system clearly rests on the ability to effectively and specifically target mRNA transcripts for degradation using anti-sense RNA. While many papers, articles, and books have been written on the subject, there are no consensus anti-sense building strategies presented. We thus chose to implement three different types of antisense inhibition: KISS, micRNA, and IS10. In the description that follows, the following nomenclature will be used:

target- the mRNA transcript that we wish to inhibit.

anti-sense- the anti-sense molecule which will bind and inhibit target.

micRNA

This anti-sense mechanism relies on two stem loops flanking an anti-sense sequence that is specific for the target. The function of the stem loops is to maintain the anti-sense region in a quasi-linear state. BBa_I1012 is built in this manner, with a linear region that will bind over the RBS, start codon, and 76 bp of BBa_I1010.

Incompatible with systems containing BBa_I1011, BBa_I1013.
Compatible with BBa_I1020, BBa_I1021, BBa_I1022, BBa_I1023.

Source

Custom design.

Sequence Annotation Location Component / Role(s)
stem_loop
stem_loop
Reverse Complement to cI mRNA
Reverse Complement RBS
reverse complement to cI cds
start
BBa_I1012
1,57
161,195
58,160
138,143
58,133
131,133
1,195
stem_loop feature/stem_loop
stem_loop feature/stem_loop
feature/misc sequence_feature
feature/misc sequence_feature
feature/misc sequence_feature
start_codon feature/start
feature/BioBrick engineered_region
igem#sampleStatus
It's complicated
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_I1012/1