Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Grace Kenney, Daniel Shen, Neelaksh Varshney, Samantha Sutton
Date created: 2003-01-31 12:00:00
Date modified: 2015-08-31 04:07:29
CI(1) IS10 asRNA
| Types | DnaRegion |
| Roles | RNA mature_transcript_region |
| Sequences | BBa_I1033_sequence (Version 1) |
Description
Region which serves as basis for transcription of asRNA that binds to and inhibitsNotes
References (unparsed) here:A synthetic oscillatory network of transcriptional regulators , Elowitz M.B. , Leibler S., Nature(403),335-38: 2000
Case, C., Roels, S., Jense, P., Lee, J., Kleckner, N. and Simons, R. (1989). The unusual stability of the IS10 anti-sense RNA is critical for its function and is determined by the structure of its stem-domain. EMBO 8(13): 4297-4305.
Jain, C. (1995). IS10 Antisense Control in Vivo is Affected by Mutations Throughout the Region of Complementarity Between the Interacting RNAs. J. Mol. Biol. 246:585-594.
Kittle, J.D., Simons, R.W., Lee, J., and Kleckner, N. (1989). Insertion Sequence IS10 Anti-sense Pairing Initiates by an Interaction Between the 5' End of the Target RNA and a Loop in the Anti-sense RNA. J. Mol. Biol. 210:561-572.
Jain, C. (1997). Models for Pairing of IS10 Encoded Antisense RNAs in vivo. J. theor. Biol. 186: 431-439.
Lutz, R., and Bujard, H. (1997). Independent and tight regulation of transcriptional units in E. coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements. Nucleic Acids Research 25(6): 1203-1210.
Ma, C., and Simons, R. (1990). The IS10 antisense RNA blocks ribosome binding at the transposase translation initiation site. EMBO 9(4):1267-1274.
E. coli codon usage table at http://bioinfo.weizmann.ac.il:3456/kegg/codon_table/codon_eco.html.
References (unparsed) here:
A synthetic oscillatory network of transcriptional regulators , Elowitz M.B. , Leibler S., Nature(403),335-38: 2000
Case, C., Roels, S., Jense, P., Lee, J., Kleckner, N. and Simons, R. (1989). The unusual stability of the IS10 anti-sense RNA is critical for its function and is determined by the structure of its stem-domain. EMBO 8(13): 4297-4305.
Jain, C. (1995). IS10 Antisense Control in Vivo is Affected by Mutations Throughout the Region of Complementarity Between the Interacting RNAs. J. Mol. Biol. 246:585-594.
Kittle, J.D., Simons, R.W., Lee, J., and Kleckner, N. (1989). Insertion Sequence IS10 Anti-sense Pairing Initiates by an Interaction Between the 5' End of the Target RNA and a Loop in the Anti-sense RNA. J. Mol. Biol. 210:561-572.
Jain, C. (1997). Models for Pairing of IS10 Encoded Antisense RNAs in vivo. J. theor. Biol. 186: 431-439.
Lutz, R., and Bujard, H. (1997). Independent and tight regulation of transcriptional units in E. coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements. Nucleic Acids Research 25(6): 1203-1210.
Ma, C., and Simons, R. (1990). The IS10 antisense RNA blocks ribosome binding at the transposase translation initiation site. EMBO 9(4):1267-1274.
E. coli codon usage table at http://bioinfo.weizmann.ac.il:3456/kegg/codon_table/codon_eco.html.
Complementary to beginning of
Can be used with all parts of the BBa_I system. It inhibits BBa_I1010. Incompatible with systems containing cI (wild type)
Source
see referencers| Sequence Annotation | Location | Component / Role(s) |
| Reverse Complement to cI mRNA stem_loop Reverse Complement RBS reverse complement to cI cds start stem_loop added codon (Cys) start LacO-1 BBa_I1033 | 56,90 79,99 68,73 56,63 61,63 58,120 58,60 56,56 1,55 1,170 | feature/misc sequence_feature feature/stem_loop stem_loop sequence_feature feature/misc feature/misc sequence_feature start_codon feature/start stem_loop feature/stem_loop feature/mutation sequence_alteration feature/start start_codon operator feature/operator engineered_region feature/BioBrick |