Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr
Date created: 2003-01-31 12:00:00
Date modified: 2015-08-31 04:07:30
Lux cassette right promoter
| Types | DnaRegion |
| Roles | promoter Regulatory |
| Sequences | BBa_I1051_sequence (Version 1) |
Description
The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the Lux repressor, LuxR. Two molecules of LuxR protein form a complex with two molecules the signalling compound homoserine lactone (HSL). This complex binds to a palindromic site on the promoter, increasing the rate of transcription.In thisversion, a binding site for cI dimer has been added: binding to this site dominantly represses transcription.Notes
Source
V. fishceri and phage lambda (courtesy of Ron Weiss)| Sequence Annotation | Location | Component / Role(s) |
| LuxR/HSL -35 -10 cI (OR1) start BBa_I1051 | 1,20 20,25 42,47 52,68 53,53 1,68 | feature/binding non_covalent_binding_site feature/promoter promoter promoter feature/promoter feature/binding non_covalent_binding_site start_codon feature/start feature/BioBrick engineered_region |