| Types | DnaRegion
|
| Roles | Regulatory
promoter
|
| Sequences | BBa_I12007_sequence (Version 1)
|
Description
Lambda Prm promoter modified to be activated but not repressed by the lambda repressor (cI)
Notes
In wild type lambda phage, the OR3 site (-27 to -11) reads "tatcccttgcggtgata" on the sense strand of DNA. To prevent lambda repressor (cI) from binding to this site, the 4th through 10th nt of OR3 were replaced by the 7 nt between OR2 and OR1, "aaatagt" (-57 to -51), which in effect mutates 5 nt of the promoter. These nt were chosen to be about halfway between the -10 and -35 boxes of the promoter. Furthermore, since these nt already act as a spacer in wild-type phage, it is hoped they will not create undesired interactions.
Source
Shih and Gussin (1983)
