Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: ryhsiao
Date created: 2004-08-18 11:00:00
Date modified: 2015-08-31 04:07:31
Modified lambda P(RM) promoter: -10 region from P(L) and cooperatively repressed by 434 cI
| Types | DnaRegion |
| Roles | Regulatory promoter |
| Sequences | BBa_I12040_sequence (Version 1) |
Description
Lamdba P(RM) promoter modified to be activated by lamda repressor (cI) and repressed by 434 repressor (cI). Two operator sites are used for both activation and repression to enhance cooperativity. Additionally, the -10 region from lambda P(L) was used instead of that from P(RM).Notes
The O-R3 site of lambda (17 nt) was replaced by the O-R1 site of 434 (14 nt) to facilitate repression by 434 cI. The new 434 O-R1 is 5'-justified with the lambda O-R3 it replaced, leaving the -10 sequence intact. The 434 O-R2 site replaces the wildtype labmda promoter starting at -5, thereby preserving the 8 nt between 434 O-R1 and O-R2 in the wildtype. The mRNA transcript will contain an additional 9 nt compared to wildtype lambda phage.This part differs from BBa_I12036 because the -10 region from P(L) was used instead of that from P(RM).
Source
Bushman, F. D. The bacteriophage 434 right operator roles of O-R1, O-R2, and O-R3. J. Mol. Biol. (1993) 230, 28-40. ; Lutz, R & Bujard, H. "Independent and tight regulation of transcriptional units in Escherichia coli via the lacR/O, the TetR/O and AraC/I1-I2 regulatory elements, Nucleic Acids Research, 1997, Vol.25, No.6 (1203-1210)| Sequence Annotation | Location | Component / Role(s) |
| OR1 lambda OR2 lambda -35 OR1 434 -10 OR2 434 start | 9,25 33,49 48,53 56,69 71,76 78,91 83,83 | feature/binding non_covalent_binding_site feature/binding non_covalent_binding_site feature/promoter promoter feature/binding non_covalent_binding_site feature/promoter promoter non_covalent_binding_site feature/binding start_codon feature/start |