BBa_I2040

BBa_I2040 Version 1

Component

Source: http://parts.igem.org/Part:BBa_I2040

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Bartholomew Canton

Date created: 2007-05-22 11:00:00

Date modified: 2015-08-31 04:07:40

lacI repressor expression device

Details

• Types: DnaRegion
• Roles: Composite; engineered_region
• Sequences: BBa_I2040_sequence (Version 1)

Description

lacI expression is controlled by the engineered, tet repressible promoter, R0040 and a medium strength RBS. A T7 transcription terminator is used downstream which is not effective for E. coli RNAP. This terminator is being used simply because the B0032.C0013.B0016 protein generator will be used in several other constructs relating to VM2.0

Notes

I'm using a T7 terminator here rather than an E. coli terminator. This is something of an accident but mainly because I want to use and understand the protein generator.

Source

n/a

Components

Access	Instance	Definition
public	BBa_R4032	BBa_R4032
public	BBa_S03691	BBa_S03691

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_R4032	1,75	BBa_R4032
BBa_S03691	82,1248	BBa_S03691

Other Properties

• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_I2040/1

Member of these Collections

• iGEM Parts Registry