Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jason Kelly
Date created: 2007-06-19 11:00:00
Date modified: 2015-08-31 04:07:41
lacZ beta-D-galactosidase [ Escherichia coli K12 ]
| Types | DnaRegion |
| Roles | Reporter engineered_region |
| Sequences | BBa_I2054_sequence (Version 1) |
Description
The lac operon is an operon required for the transport and metabolism of lactose in Escherichia coli and some other enteric bacteria. It consists of three adjacent structural genes, a promoter, a terminator, and an operator. The lac operon is regulated by several factors including the availability of glucose and of lactose. Gene regulation of the lac operon was the first genetic regulatory mechanism to be elucidated and is often used as the canonical example of prokaryotic gene regulation. LacZ encodes (LacZ), an intracellular enzyme that cleaves the disaccharide lactose into glucose and galactose. The lac gene and its derivatives are amenable to use as a reporter gene in a number of bacterial-based selection techniques such as two hybrid analysis, in which the successful binding of a transcriptional activator to a specific promoter sequence must be determined.[2] In LB plates containing X-gal, the colour change from white colonies to a shade of blue, corresponds to about 20-100 β-galactosidase units, while tetrazolium lactose and MacConkey lactose media have a range of 100-1000 units, being most sensitive in the high and low parts of this range respectively. [2] Since MacConkey lactose and tetrazolium lactose media both rely on the products of lactose breakdown, they require the presence of both lacZ and lacY genes. The many lac fusion techniques which include only the lacZ gene are thus suited to the X-gal plates [2] or ONPG liquid broths [1]Source
http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?val=NC_000913.2&from=362455&to=365529&strand=2&dopt=gbLocation/Qualifiers
source 1..3075
/organism="Escherichia coli K12"
/mol_type="genomic DNA"
/strain="K-12"
/sub_strain="MG1655"
/db_xref="taxon:83333"
gene 1..3075
/gene="lacZ"
/locus_tag="b0344"
/note="synonyms: ECK0341, JW0335"
/db_xref="ECOCYC:EG10527"
/db_xref="EcoGene:EG10527"
/db_xref="GeneID:945006"