pUC19 ori

BBa_I50022 Version 1

Component

Source: http://parts.igem.org/Part:BBa_I50022

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Reshma Shetty

Date created: 2008-01-05 12:00:00

Date modified: 2015-08-31 04:07:42

Minimal pUC19-derived high copy replication origin

Details

• Types: DnaRegion
• Roles: sequence_feature; DNA
• Sequences: BBa_I50022_sequence (Version 1)

Description

High copy origin of replication derived from pSB1A3. Designed to omit antibiotic resistance markers and primer binding sites. Lacks mutations that render BBa_I50020 nonfunctional as a replication origin.

Notes

We had to leave several restriction sites in because point mutations that eliminated these sites were found to render the origin nonfunctional.

Source

pSB1A3

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
rep (pMB1)	16,630	sequence_feature, feature/misc
RNAII transcript promoter -35 sequence	27,32	feature/promoter, promoter
origin of replication	27,615	feature/misc, sequence_feature
RNAII transcript promoter -10 sequence	48,53	feature/promoter, promoter
RNAII transcript	63,615	feature/misc, sequence_feature
RNAI transcript	66,173	sequence_feature, feature/misc
RNAI transcript promoter -10	182,187	promoter, feature/promoter
RNAI transcript promoter -35	204,209	promoter, feature/promoter
ORI	615,615	sequence_feature, feature/misc

Other Properties

• igem#experience: Works
• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_I50022/1

Member of these Collections

• //plasmid/component/origin
• iGEM Parts Registry