BBa_I50030

This part is the replication origin from plasmid pBR322 which is supposed to have low to medium copy number.

Sequence Annotation	Location	Component / Role(s)
pMB1 origin of replication repeat region found in Tn3 RNAII transcript promoter -35 sequence RNAII transcript promoter -10 sequence RNAI transcript RNAI transcript promoter -10 sequence RNAI transcript promoter -35 sequence rep pMB1 RNAII transcript replication origin L-strand Y effector site gamma-delta insertion target sequence H-strand Y effector site RBS 1 RBS 2 gamma-delta insertion target sequence from pSC101 gamma-delta insertion target sequence	171,759 108,145 171,176 192,197 210,317 326,331 348,353 160,774 207,759 758,758 879,942 1044,1048 1126,1282 1383,1388 1384,1388 1501,1505 1531,1657 1774,1778	sequence_feature feature/misc sequence_feature feature/misc feature/promoter promoter feature/promoter promoter sequence_feature feature/misc feature/promoter promoter feature/promoter promoter feature/misc sequence_feature sequence_feature feature/misc sequence_feature feature/misc feature/misc sequence_feature sequence_feature feature/misc feature/misc sequence_feature feature/rbs ribosome_entry_site feature/rbs ribosome_entry_site feature/misc sequence_feature sequence_feature feature/misc sequence_feature feature/misc

//plasmid/component/origin iGEM Parts Registry