BBa_I712028

BBa_I712028 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_I712028
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Marko Bitenc
Date created: 2007-10-20 11:00:00
Date modified: 2015-08-31 04:07:46

CherryNLS - synthetic construct monomeric red fluorescent protein with nuclear localization sequence



Types
DnaRegion

Roles
CDS

Coding

Sequences BBa_I712028_sequence (Version 1)

Description

This protein is used to show that T7 polymerase is actually going into the nucleolus, where it can transcribe genes under T7 promotor.

Notes

In the original cherry protein there was a PstI restriction site in the middle of the sequence. That is why mutagenesis of this restiction site was necessary.

Source

From one clone that Gabi gave me - will be further described in the future.

igem#experience
Works
 
igem#sampleStatus
It's complicated
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_I712028/1