BBa_I712070

BBa_I712070 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_I712070
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Sasa Jereb
Date created: 2007-10-22 11:00:00
Date modified: 2015-08-31 04:07:46

T7 promoter - RBS -T7 RNA polymerase with NLS



Types
DnaRegion

Roles
engineered_region

Composite

Sequences BBa_I712070_sequence (Version 1)

Description

T7 RNA polymerase with RBS and NLS (nuclear localisation signal) under T7 promoter. This construct was designed for self-amplification of T7 RNA polymerase.

Notes

T7 RNA polymerase gene was isolated from BL21 and PCR was performed to attach NLS and RBS.

Source

Genomic DNA of BL21 (E.coli strain)

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BBa_I712070/1