Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Xili Liu
Date created: 2007-10-24 11:00:00
Date modified: 2015-08-31 04:07:49
pCC002
| Types | DnaRegion |
| Roles | Plasmid plasmid |
| Sequences | BBa_I714946_sequence (Version 1) |
Description
with a Ptet promoter controled by TetR, a MCS, T1 terminator, p15A ori and Cm resistence gene. copy number: 15~20Notes
to measure the efficiency of different SD signal, the plasmid has a MCS downstream of Ptet where can insert reporters along with SD signal. There is a T1 terminator downstream of MCS to terminate transcription starting from Ptet. Ptet is repressed by TetR carried on this plasmid and can be turned on by adding aTc.Source
pCRT-1| Sequence Annotation | Location | Component / Role(s) |
| promoter of cat R0040 MCS B0010 p15A ori C0040 cat | 74,103 130,177 224,286 298,388 431,1179 1279,1909 2277,2936 | feature/promoter promoter feature/BioBrick engineered_region feature/dna sequence_feature feature/BioBrick engineered_region feature/conserved conserved_region engineered_region feature/BioBrick CDS feature/protein |