BBa_I716008

BBa_I716008 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_I716008
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Arthur Yu
Date created: 2007-06-27 11:00:00
Date modified: 2015-08-31 04:07:50

Ptet-rbs-wbbL-rbs-neuS



    • Details

    Types
    DnaRegion

    Roles
    engineered_region

    Composite

    Sequences BBa_I716008_sequence (Version 1)

    Description

    A working operon, made with rbs libraries, in order to test the activity of wbbL and neuS together.

    Notes

    {{BerkiGEM2007-Biobrick2.0Intro}}


    
Let's make I716008 (Ptet-rbs-wbbL-rbs-neuS)
    
[digest] pcr prod of Ptet (EcoRI/BamHI, 69+6, L)
    
[digest] pBca1122-Bca9270 (EcoRI/BglII, 1027+4140, L)
    
[ligate]
    
[prod] I716008 (Ptet-rbs-wbbL-rbs-neuS)
    

    

    
---
    
(pcr prod) Ptet gaattcatgAGATCTtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcacGGATCC


    Source

    From the basic parts I716000+I716001, rbs libraries 1121-9236, 1122-9236, and a PCR product of Ptet promoter.

    igem#sampleStatus
    Not in stock
    igem#status
    Unavailable
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_I716008/1
     

    Attachments

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