BBa_I716020

BBa_I716020 Version 1

Component

Source: http://parts.igem.org/Part:BBa_I716020

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Arthur Yu

Date created: 2007-07-23 11:00:00

Date modified: 2015-08-31 04:07:50

(pBca1176) (RFPcass-CmR-colE1) on I716101

Details

• Types: DnaRegion
• Roles: Composite; engineered_region
• Sequences: BBa_I716020_sequence (Version 1)

Description

this makes the low copy I716101 hi copy (colE1) and adds a CAM resistance to allow selection for the good clones. Therefore, the low copy is now high copy and easier to work with; we can excise the cam resistance and the colE1 once the other things are cloned in.

Notes

PCR ca1176F/R on pSB1A2-Bca1016     (1798 bp, BamHI/XhoI/DpnI) 

Sub into I716101                    (BamHI/XhoI, 4051+9, L)

Product is pBca1176 (Select on Cam/Amp)



ca1176F    Forward BamHI for CmR/colE1    gctctGGATCCggaataggaacttcatttaaatg 

ca1176R    Reverse XhoI for CmR/ColE1    cgtaaCTCGAGaagatcctttttgataatc

{{BerkiGEM2007-Biobrick2.0Intro}}

Source

See design

Other Properties

• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_I716020/1

Member of these Collections

• iGEM Parts Registry