Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Arthur Yu
Date created: 2007-08-02 11:00:00
Date modified: 2015-08-31 04:07:50
p-mgrB Mg repressed promoter
| Types | DnaRegion |
| Roles | sequence_feature Other |
| Sequences | BBa_I716028_sequence (Version 1) |
Description
Mg2+ repressed promotersee
"Molecular Characterization of the PhoP-PhoQ Two-Component
System in Escherichia coli K-12: Identification of Extracellular
Mg21-Responsive Promoters"
AKINORI KATO, HIROYUKI TANABE, AND RYUTARO UTSUMI*
Notes
{{BerkiGEM2007-Biobrick2.0Intro}}
making basic biobrick I716028 - Mg2+ repressed promoter (p-mgrB)
[pcr] AY021/22 onto MG1655 genome (550 bp, BglII/XhoI)
put it into pBca9145
you get I716028
mgrB attempt by yu (550 bp)
AY021 (f) catggAGATCTtgagagtaagaacctgtcgg
AY022 (r1) gtacaCTCGAGttaGGATCCgtttcacctaccttatgtc