BBa_I716029

BBa_I716029 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_I716029
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Arthur Yu
Date created: 2007-08-02 11:00:00
Date modified: 2015-08-31 04:07:50

mgrB - rbs and stops at ATG



    • Details

    Types
    DnaRegion

    Roles
    Other

    sequence_feature

    Sequences BBa_I716029_sequence (Version 1)

    Description

    see I716028

    this is like that, except it stops right before ATG in the MG1655 genome.

    Notes

    {{BerkiGEM2007-Biobrick2.0Intro}}

    
making basic biobrick I716029 - Mg2+ repressed promoter (p-mgrB)
    

    
[pcr] AY021/23 onto MG1655 genome   (550 bp, BglII/XhoI)
    
put it into pBca9145
    
you get I716029
    

    

    
mgrB attempt by yu (550 bp)
    
AY021 (f)  catggAGATCTtgagagtaagaacctgtcgg 
    
AY023 (r2) gtacaCTCGAGttaGGATCCcgaaactttttcactccaatc


    Source

    K12 MG1655

    igem#sampleStatus
    Not in stock
    igem#status
    Unavailable
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_I716029/1
     

    Attachments

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