Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Arthur Yu
Date created: 2007-08-02 11:00:00
Date modified: 2015-08-31 04:07:50
mgrB - rbs and stops at ATG
| Types | DnaRegion |
| Roles | Other sequence_feature |
| Sequences | BBa_I716029_sequence (Version 1) |
Description
see I716028this is like that, except it stops right before ATG in the MG1655 genome.
Notes
{{BerkiGEM2007-Biobrick2.0Intro}}
making basic biobrick I716029 - Mg2+ repressed promoter (p-mgrB)
[pcr] AY021/23 onto MG1655 genome (550 bp, BglII/XhoI)
put it into pBca9145
you get I716029
mgrB attempt by yu (550 bp)
AY021 (f) catggAGATCTtgagagtaagaacctgtcgg
AY023 (r2) gtacaCTCGAGttaGGATCCcgaaactttttcactccaatc