Types | DnaRegion
|
Roles | Composite
engineered_region
|
Description
2X mutant -pTRC-rbs-beta-rbs-alpha L29F/V96W and Asn108Lys/Lys82Asp
Notes
{{BerkiGEM2007-Biobrick2.0Intro}}
Source
p-TRC (Bgl2/Xho1, 4136/1713, Larger)
I716067 -Kan-rbs-beta-rbs-alpha (Bgl2/Xho1, 3081/970, Smaller)
Ligated Product is I716069 2X mutant -pTRC-rbs-beta-rbs-alpha (Select the [hopefully] bloodly looking colonies)