BBa_I716209

BBa_I716209 Version 1

Component

Source: http://parts.igem.org/Part:BBa_I716209

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Samantha Liang

Date created: 2007-06-04 11:00:00

Date modified: 2015-08-31 04:07:51

ceaB

Details

• Types: DnaRegion
• Roles: CDS; Coding
• Sequences: BBa_I716209_sequence (Version 1)

Description

colicin activity protein - Dnase for bacteria self-destruct mechanism
without a start codon so inactive

Notes

PCR sil02F and sil02R on colE2 colicin region (1758bp, BglII/XhoI)
Sub into pBca9145-Bca1089 (BglII/XhoI, 2063+696, L)
Product is pBca9145-I716209

sil02F Forward BglII biobrick of ceaB ccaaaAGATCTagcggtggcgatggacgcgg
sil02R Reverse XholI/BamHI biobrick of ceaB gctagCTCGAGttaGGATCCttacttaccccgatgaatatc

Source

PCR sil02F and sil02R on colE2 colicin region (1758bp, BglII/XhoI)
Sub into pBca9145-Bca1089 (BglII/XhoI, 2063+696, L)
Product is pBca9145-I716209

sil02F Forward BglII biobrick of ceaB ccaaaAGATCTagcggtggcgatggacgcgg
sil02R Reverse XholI/BamHI biobrick of ceaB gctagCTCGAGttaGGATCCttacttaccccgatgaatatc

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_I716209/1

Member of these Collections

• //cds/enzyme/dnase
• iGEM Parts Registry