Types | DnaRegion
|
Roles | Coding
CDS
|
Sequences | BBa_I716211_sequence (Version 1)
|
Description
Barnase active peptide (no start codon)
Notes
PCR sil03F and sil03R on barnase region (348 bp, BglII/XhoI)
Sub into pBca9145-Bca1089 (BglII/XhoI, 2063+696, L)
Product is pBca9145-I716211
sil03F Forward BglII biobrick of barnase ccaaaAGATCTgcacaggttatcaacacgtttg
sil03R Reverse XholI/BamHI biobrick of barnase gctagCTCGAGttaGGATCCttatctgatttttgtaaagg
Source
PCR sil03F and sil03R on barnase region (348 bp, BglII/XhoI)
Sub into pBca9145-Bca1089 (BglII/XhoI, 2063+696, L)
Product is pBca9145-I716211
sil03F Forward BglII biobrick of barnase ccaaaAGATCTgcacaggttatcaacacgtttg
sil03R Reverse XholI/BamHI biobrick of barnase gctagCTCGAGttaGGATCCttatctgatttttgtaaagg