BBa_I716211

BBa_I716211 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_I716211
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Samantha Liang
Date created: 2007-06-04 11:00:00
Date modified: 2015-08-31 04:07:51

Barnase active peptide



Types
DnaRegion

Roles
Coding

CDS

Sequences BBa_I716211_sequence (Version 1)

Description

Barnase active peptide (no start codon)

Notes

PCR sil03F and sil03R on barnase region (348 bp, BglII/XhoI)
Sub into pBca9145-Bca1089 (BglII/XhoI, 2063+696, L)
Product is pBca9145-I716211

sil03F Forward BglII biobrick of barnase ccaaaAGATCTgcacaggttatcaacacgtttg
sil03R Reverse XholI/BamHI biobrick of barnase gctagCTCGAGttaGGATCCttatctgatttttgtaaagg

Source

PCR sil03F and sil03R on barnase region (348 bp, BglII/XhoI)
Sub into pBca9145-Bca1089 (BglII/XhoI, 2063+696, L)
Product is pBca9145-I716211

sil03F Forward BglII biobrick of barnase ccaaaAGATCTgcacaggttatcaacacgtttg
sil03R Reverse XholI/BamHI biobrick of barnase gctagCTCGAGttaGGATCCttatctgatttttgtaaagg

igem#experience
None
 
igem#partStatus
Released HQ 2013
igem#sampleStatus
In stock
igem#status
Available
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_I716211/1