BBa_I716212

BBa_I716212 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_I716212
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Samantha Liang
Date created: 2007-06-04 11:00:00
Date modified: 2015-08-31 04:07:51

Cre (GTG start)



    • Details

    Types
    DnaRegion

    Roles
    Coding

    CDS

    Sequences BBa_I716212_sequence (Version 1)

    Description

    weakened version of Cre (has a GTG start codon) because we want a lower copy

    Notes

    PCR sil05 and G00101 on pBca9145-Bca1117 (PCR product is 1147bp, BglII/XhoI 1047 bp)
    Sub into pBca9145-Bca1089 (BglII/XhoI, 2063+696, L)
    Product is pBca9145-I716212 (GTG start)

    sil05 Forward BglII Cre with GTG start ctagcAGATCTgtgtccaatttactgaccgtac
    G00101 Reverse Sequencing of pSB1A* plasmids attaccgcctttgagtgagc

    Source

    PCR sil05 and G00101 on pBca9145-Bca1117 (PCR product is 1147bp, BglII/XhoI 1047 bp)
    Sub into pBca9145-Bca1089 (BglII/XhoI, 2063+696, L)
    Product is pBca9145-I716212 (GTG start)

    sil05 Forward BglII Cre with GTG start ctagcAGATCTgtgtccaatttactgaccgtac
    G00101 Reverse Sequencing of pSB1A* plasmids attaccgcctttgagtgagc

    igem#experience
    None
     
    igem#sampleStatus
    It's complicated
    igem#status
    Planning
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_I716212/1
     

    Attachments

    © 2018 Newcastle University, University of Utah, and collaborators
    About SynBioHub | View Source on Github | Report an Issue
    | v1.6.1 (86615526)