Types | DnaRegion
|
Roles | sequence_feature
Other
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Sequences | BBa_I716252_sequence (Version 1)
|
Description
basic part for the chaperone/toxicity device
Notes
{{BerkiGEM2007-Biobrick2.0Intro}}
Construction of a Biobrick 2.0 basic part in plasmid pBca9145-Bca1144 (Clone # 5) for the SodC open reading frame from Escherichia coli K-12 MG1655
PCR VU01F.sodC/VU02R.sodC on SodC (553 bp, BglII/XhoI)
Digest pBca9145-Bca1144 (Clone # 5) (BglII/XhoI, 2063+910, L)
Product is pBca9145-VU1
-------------------------------------
VU01F.sodC ??? ccaaaAGATCTatgaaacgttttagtctggc
VU02R.sodC ??? gctagCTCGAGttaGGATCCttacttaattacaccacaggc
Source
pBca9145-Bca1144 (Clone # 5)