BBa_I716254

BBa_I716254 Version 1

Component

Source: http://parts.igem.org/Part:BBa_I716254

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Vaibhavi Umesh

Date created: 2007-06-05 11:00:00

Date modified: 2015-08-31 04:07:51

N-terminal aminopeptidase (methionine aminopeptidase ??? map gene)

Details

• Types: DnaRegion
• Roles: Coding; CDS
• Sequences: BBa_I716254_sequence (Version 1)

Description

basic part for the chaperone/toxicity device

Notes

{{BerkiGEM2007-Biobrick2.0Intro}}

N-terminal aminopeptidase (methionine aminopeptidase ??? map gene)

Construction of a Biobrick 2.0 basic part in plasmid pBca9145-Bca1144 (Clone # 5) for the N-terminal methionine aminopeptidase (map) open reading frame from Salmonella typhimurium LT2

PCR VU07F.map/VU08R.map on methionine aminopeptidase (817 bp, BglII/XhoI)
Digest pBca9145-Bca1144 (Clone # 5) (BglII/XhoI, 2063+910, L)
Product is pBca9145-VU3
-------------------------------------
VU07F.map ccaaaAGATCTatggctatctcaatcaagacccc
VU08R.map gctagCTCGAGttaGGATCCttattcgtcgtgcgagattatcg

Source

Escherichia coli K-12 MG1655

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• rdf-schema#seeAlso: P0AE18
• rdf-schema#seeAlso: P0AE19
• rdf-schema#seeAlso: P0AE20
• rdf-schema#seeAlso: P0AE21
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_I716254/1

Member of these Collections

• //cds/enzyme
• iGEM Parts Registry