Types | DnaRegion
|
Roles | engineered_region
Composite
|
Sequences | BBa_I716306_sequence (Version 1)
|
Description
T7 promoter added in front of RBS site(aggagg).
Notes
Biobrickificreation of T7 Promoters I716105
PCR ca1090F and dt008-PT7-wt (62 bp, EcoRI/BamHI, Product = A)
Sub into pBca9145-Bca1144 (EcoRI/BamHI, 2063+910, L)
Product A is pBca9145-I716105-PT7-wt
dt008-PT7-wt T7 Promoter (Wild Type) GtttGGATCCaccTCTCCCTATAGTGAGTCGTATTAtacAGATCTcatGAATTCcagaa
Sub into pJD507_pSB1A2C (EcoRI, BglII 2071bp, 9bp L)
Produst is I716306
Source
{{BerkiGEM2007-Biobrick2.0Intro}}