BBa_I716306

BBa_I716306 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_I716306
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: vincent parker
Date created: 2007-07-23 11:00:00
Date modified: 2015-08-31 04:07:52

T7-RBS



    • Details

    Types
    DnaRegion

    Roles
    engineered_region

    Composite

    Sequences BBa_I716306_sequence (Version 1)

    Description

    T7 promoter added in front of RBS site(aggagg).

    Notes

    Biobrickificreation of T7 Promoters I716105

    PCR ca1090F and dt008-PT7-wt (62 bp, EcoRI/BamHI, Product = A)
    Sub into pBca9145-Bca1144 (EcoRI/BamHI, 2063+910, L)
    Product A is pBca9145-I716105-PT7-wt

    dt008-PT7-wt T7 Promoter (Wild Type) GtttGGATCCaccTCTCCCTATAGTGAGTCGTATTAtacAGATCTcatGAATTCcagaa

    Sub into pJD507_pSB1A2C (EcoRI, BglII 2071bp, 9bp L)
    Produst is I716306

    Source

    {{BerkiGEM2007-Biobrick2.0Intro}}

    igem#sampleStatus
    Not in stock
    igem#status
    Unavailable
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_I716306/1
     

    Attachments

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