BBa_I716306

BBa_I716306 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_I716306
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: vincent parker
Date created: 2007-07-23 11:00:00
Date modified: 2015-08-31 04:07:52

T7-RBS



Types
DnaRegion

Roles
engineered_region

Composite

Sequences BBa_I716306_sequence (Version 1)

Description

T7 promoter added in front of RBS site(aggagg).

Notes

Biobrickificreation of T7 Promoters I716105


PCR ca1090F and dt008-PT7-wt (62 bp, EcoRI/BamHI, Product = A)
Sub into pBca9145-Bca1144 (EcoRI/BamHI, 2063+910, L)
Product A is pBca9145-I716105-PT7-wt


dt008-PT7-wt T7 Promoter (Wild Type) GtttGGATCCaccTCTCCCTATAGTGAGTCGTATTAtacAGATCTcatGAATTCcagaa


Sub into pJD507_pSB1A2C (EcoRI, BglII 2071bp, 9bp L)
Produst is I716306

Source

{{BerkiGEM2007-Biobrick2.0Intro}}

igem#sampleStatus
Not in stock
igem#status
Unavailable
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_I716306/1