BBa_I716375

BBa_I716375 Version 1

Component

Source: http://parts.igem.org/Part:BBa_I716375

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Austin Day

Date created: 2007-10-24 11:00:00

Date modified: 2015-08-31 04:07:52

Biobricked Mutant Hemoglobin Cassette in petDUET format with hemABCD

Details

• Types: DnaRegion
• Roles: engineered_region; Composite

Description

This part consists of the beta and alpha subunits of human hemoglobin A inserted into the commercial petDUET vector. The hemoglobin subunits are mutants with the following mutations: beta providence (Asn108Lys) and presbyterian (Lys82Asp) and the alpha subunit was made into a di-alpha fusion with a glycine linker between the two subunits.

The second part consists of the genes in the heme biosynthesis pathway hemA, hemB, hemC, and hemD.

Notes

{{BerkiGEM2007-Biobrick2.0Intro}}

Source

Digest I716356 (4008/9, BamH1/Xho1, Larger)

Digest I716390 (4219/2054/9, BglII/XhoI, Largest)

1,2,3 Product is I716375

Other Properties

• igem#sampleStatus: No part sequence
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_I716375/1

Member of these Collections

• iGEM Parts Registry