BBa_I716376

BBa_I716376 Version 1

Component

Source: http://parts.igem.org/Part:BBa_I716376

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Austin Day

Date created: 2007-10-24 11:00:00

Date modified: 2015-08-31 04:07:52

Biobricked Hemoglobin Cassette in petDUET format with hemABCD with cytochromes

Details

• Types: DnaRegion
• Roles: Composite; engineered_region

Description

This part consists of the beta and alpha subunits of human hemoglobin A inserted into the commercial petDUET vector. The hemoglobin subunits are mutants with the following mutations: beta providence (Asn108Lys) and presbyterian (Lys82Asp) and the alpha subunit was made into a di-alpha fusion with a glycine linker between the two subunits.

The second part consists of the genes in the heme biosynthesis pathway hemA, hemB, hemC, and hemD.

The third part consists of the genes encoding for cytochrome b5 and cytochrome b5 reductase.

Notes

{{BerkiGEM2007-Biobrick2.0Intro}}

Source

Digest I716374 (BamHI/XhoI, 7651/9, Larger)

Digest I716019 (BglII/XhoI, 2063/1401, Smaller)

1,2,3 Product is I716376

Other Properties

• igem#sampleStatus: No part sequence
• igem#status: Unavailable
• synbiohub#ownedBy: user/myers
• synbiohub#ownedBy: user/james
• synbiohub#topLevel: BBa_I716376/1

Member of these Collections

• iGEM Parts Registry