Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Austin Day
Date created: 2007-10-24 11:00:00
Date modified: 2015-08-31 04:07:52
Biobricked Mutant Hemoglobin Cassette in petDUET format with hemABCD with AHSP
| Types | DnaRegion |
| Roles | engineered_region Composite |
Description
This part consists of the beta and alpha subunits of human hemoglobin A inserted into the commercial petDUET vector. The hemoglobin subunits are mutants with the following mutations: beta providence (Asn108Lys) and presbyterian (Lys82Asp) and the alpha subunit was made into a di-alpha fusion with a glycine linker between the two subunits.The second part consists of the genes in the heme biosynthesis pathway hemA, hemB, hemC, and hemD.
The third part consists of the gene that encodes for human alpha hemoglobin stabilizing protein.
Notes
{{BerkiGEM2007-Biobrick2.0Intro}}Source
Digest I716374 (BamHI/XhoI, 7651/9, Larger)Digest I716276 (BglII/XhoI, 2057/542, Smaller)
1,2,3 Product is I716375