pTG262

BBa_I742103 Version 1

Component

Source: http://parts.igem.org/Part:BBa_I742103

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: sarah hollingshead

Date created: 2007-10-05 11:00:00

Date modified: 2015-08-31 04:08:02

pTG262 Plasmid (Gram negative to Gram positive shuttle vector)

Details

• Types: DnaRegion
• Roles: plasmid; Plasmid
• Sequences: BBa_I742103_sequence (Version 1)

Description

Plasmid contains an origin of replication which is capable of replicating in E. coli and several gram positive organisms including Lactobacillus, Lactococcus and Bacillus. Plasmid has chloramphenicol, neomycin & gentamicin resistance genes. It also has a EcoRI and PstI site with an XbaI site situated in the middle so biobricks can be inserted.

Gene instability may occur with certain inserts (we have not experienced this so far with the biobricks we have inserted).

Notes

Plasmid had to be able to act as a shuttle vector between E. coli and Lactobacillus, replicated in both organisms and have suitable resistance genes and stability

Source

Kindly donated by Dr Mike Gasson & Dr. Claire Shearman, of the Institute of Food Research in Norwich, United Kingdom

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
cat	917,1557	feature/cds, CDS
PstI	424,429	sequence_feature, feature/misc
XbaI	436,441	feature/misc, sequence_feature
EcoRI	463,468	feature/misc, sequence_feature
SacI	457,462	sequence_feature, feature/misc
neo	2092,2862	CDS, feature/cds
repA	3580,4287	feature/cds, CDS

Other Properties

• igem#experience: Works
• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_I742103/1

Member of these Collections

• iGEM Parts Registry