LIMS1

BBa_I742110 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_I742110
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: sarah hollingshead
Date created: 2007-10-18 11:00:00
Date modified: 2015-08-31 04:08:02

LIMS1



Types
DnaRegion

Roles
Coding

CDS

Sequences BBa_I742110_sequence (Version 1)

Description

Limonene synthase 1 from Citrus limon (accession AF514287)
Synthesised by Geneart.

Limonene synthase converts farnesyl-diphosphate to + limonene, which is a component of lemon scent.

Notes

several codons were altered silently to remove several forbidden restriction sites. The N-terminal region (plastid targeting sequence) was removed, removing the first 51 amino acids and replacing codon 52 with the start codon ATG

Source

Monoterpene biosynthesis in lemon (Citrus limon) cDNA isolation and functional analysis of four monoterpene synthases, J Lucker, et al, Eur. J. Biochem. 269: 3160???3171 (2002)

Analysis of Lemon and Bergamot Essential Oils by HPLC with Microbore Columns, M Benincasa, et al, Chromatographia 30(5/6):271 (1990)

Citrus fruit flavour and aroma biosynthesis, L Sharon-Asa, The Plant Journal, 36:664 (2003)

Sequence Annotation Location Component / Role(s)
(+) limonene synthase coding region
start
stop
1,1671
1,3
1666,1671
feature/cds CDS
feature/start start_codon
stop_codon feature/stop
igem#experience
None
 
igem#sampleStatus
Not in stock
igem#status
Unavailable
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_I742110/1