Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: sarah hollingshead
Date created: 2007-10-18 11:00:00
Date modified: 2015-08-31 04:08:02
enoyl CoA hydratase (ech) gene
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_I742112_sequence (Version 1) |
Description
enoyl CoA hydratase (ech) was isolated from Pseudomonas fluorescens genome.Ech converts feruloyl CoA to vanillin (fig. 3) and catalyses the final stage of the vanillin biosynthesis pathway.
Involved in the synthesis of 'natural' vanilla flavouring
Notes
Gene was synthesised by geneart as we experienced problems isolating native gene, due to the high GC nature of the Pseudomonas genome.The ech sequence has been optimised for expression in E. coli
Source
Pseudomonas fluorescens NCIMB 9046Cloning and Characterization of the Ferulic Acid Catabolic Genes of Sphingomonas paucimobilis SYK-6, E Masai, et al, APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 68(9): 4416???4424 (2002)