BBa_I742114

BBa_I742114 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_I742114
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: sarah hollingshead
Date created: 2007-10-18 11:00:00
Date modified: 2015-08-31 04:08:02

Feruloyl-CoA synthase (fcs)



Types
DnaRegion

Roles
Coding

CDS

Sequences BBa_I742114_sequence (Version 1)

Description

gene is from Pseudomonas fluorescens

encodes feruoyl CoA synthase (ligates acetyl-CoA onto ferulic acid)

produces feruloyl CoA from ferrulic acid

catalyses the penultimate of the vanillin biosynthesis pathway

Notes

Gene was synthesised by geneart, as P. fluorescens has a high GC genome.

Coding region was optimised for gene expression in E. coli

Source

Pseudomonas fluorescens NCIMB 9046

Cloning and Characterization of the Ferulic Acid Catabolic Genes of Sphingomonas paucimobilis SYK-6, E Masai, et al, APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 68(9): 4416???4424 (2002)

Sequence Annotation Location Component / Role(s)
fcs coding region
1,1773
CDS feature/cds
igem#sampleStatus
Not in stock
igem#status
Unavailable
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_I742114/1