BBa_I742116

BBa_I742116 Version 1

Component

Source: http://parts.igem.org/Part:BBa_I742116

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: sarah hollingshead

Date created: 2007-10-18 11:00:00

Date modified: 2015-08-31 04:08:02

crtE with native ribosome binding site

Details

• Types: DnaRegion
• Roles: CDS; Coding
• Sequences: BBa_I742116_sequence (Version 1)

Description

CrtE encodes geranylgeranyl pyrophosphate synthase

Enzyme converts two farnesyl diphosphate molecules into geranylgeranyl diphosphate

Part of the carotenoid synthesis pathway

Notes

Ensured gene contained ribosome binding site by designing primers to include the site

Source

Diversity of Carotenoid Synthesis Gene Clusters from Environmental Enterobacteriaceae Strains, N Sedkova, APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 71(12): 8141???8146 (2005)

Elucidation of Pathway the Erwinia uredovora Carotenoid Biosynthetic by Functional Analysis of Gene Products Expressed in Escherichia coli, N Misawa, et al, JOURNAL OF BACTERIOLOGY, 172(12): 6704-6712 (1990)

Pantoea ananatis (formerly Erwinia uredovora) DSMZ 30080 (ATCC 19321).
Accession: D90087

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
CrtE coding region	16,954	CDS, feature/cds
native rbs	1,15	ribosome_entry_site, feature/rbs

Other Properties

• igem#experience: Works
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_I742116/1

Member of these Collections

• iGEM Parts Registry