Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: sarah hollingshead
Date created: 2007-10-18 11:00:00
Date modified: 2015-08-31 04:08:02
crtIB with both native ribosome binding sites
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_I742119_sequence (Version 1) |
Description
phytoene dehydrogenase and phytoene synthase from Pantoea ananatisphytoene dehydrogenase produced Lycopene from phytoene
Lycopene is a red pigment with antioxident properties
Phytoene synthase produced phytoene from geranylgeranyl diphosphate
Both enzymes are part of the carotenoid biosynthesis pathway
Notes
The crtI coding sequence contains 2 PstI sites. These have been removed by site-directed mutagenesis but the functionality of the nutated gene has not been confirmed.This version still contains the two PstI sites.
Source
from Pantoea ananatis (formerly Erwinia uredovora) DSMZ 30080 (ATCC 19321)Diversity of Carotenoid Synthesis Gene Clusters from Environmental Enterobacteriaceae Strains, N Sedkova, APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 71(12): 8141???8146 (2005)
Elucidation of Pathway the Erwinia uredovora Carotenoid Biosynthetic by Functional Analysis of Gene Products Expressed in Escherichia coli, N Misawa, et al, JOURNAL OF BACTERIOLOGY, 172(12): 6704-6712 (1990)