| Types | DnaRegion
|
| Roles | CDS
Coding
|
| Sequences | BBa_I742137_sequence (Version 1)
|
Description
Integral membrane-protein FtsK translocates to the septum at bacterial division, clutches DNA and tugs at it so that replicated DNA monomers that are interdigitated may be excised by the XerCD recombinase complex. See Edinburgh 2007 project background for more detail.
Notes
The TC bases at the very beginning of the sequence are there to accomodate a SacI restriction site. The FtsK sequence naturally contains one NotI and two PstI restriction sites. These will need to be removed before it can feature as a 'real' biobrick in future projects.
Source
FtsK is amplified straight from the E. coli K12 genome.
