RQE

BBa_I744104 Version 1

Component

Source: http://parts.igem.org/Part:BBa_I744104

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Thomas Segall-Shapiro

Date created: 2007-07-17 11:00:00

Date modified: 2015-08-31 04:08:03

tetracycline sensor with ptet promoter

Details

• Types: DnaRegion
• Roles: Measurement; engineered_region
• Sequences: BBa_I744104_sequence (Version 1)

Description

This biobrick is intended to report on the levels of Tetracycline inside a cell. It uses part R0040 (the tet promoter) to drive synthesis of tetR.

Notes

The promoter driving tetR production is crucial to the sensor's sensitivity and range of detection. In this sensor, the tet promoter creates a negative feedback loop, which should keep levels of tetR reasonable even with high copy number plasmids.

Source

Composite - all parts are taken from the registry

Components

Access	Instance	Definition
public	BBa_R0040	p(tetR)
public	BBa_Q04400	tetR
public	BBa_E0430	BBa_E0430

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_R0040	1,54	p(tetR)
BBa_Q04400	63,964	tetR
BBa_E0430	973,1850	BBa_E0430

Other Properties

• igem#experience: Works
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_I744104/1

Member of these Collections

• iGEM Parts Registry