N2C1

BBa_I744211 Version 1

Component

This part has been discontinued.

Source:
http://parts.igem.org/Part:BBa_I744211
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Peter Nguyen
Date created: 2007-10-23 11:00:00
Date modified: 2015-08-31 04:08:03

LuxN-Tsr Chimeric Receptor C



Types
DnaRegion

Roles
CDS

Coding

Sequences BBa_I744211_sequence (Version 1)

Description

TetR-promoter driven chimeric receptor containing the N-terminal transmembrane sensor domain (1..442) of the quorum-sensing receptor LuxN from Vibrio harveyi fused to the C-terminal signaling domain (260..551) of the E.coli MCP receptor, Tsr. The domains are linked by an NdeI site (translation = 'HM').

Source

LuxN N-terminal domain is from Vibrio harveyi. Tsr C-terminal domain is from E.coli.

Sequence Annotation Location Component / Role(s)
Start translation
Stop translation
LuxN sensory domain fragment 2
Tsr signaling domain fragment 1
1,3
2209,2211
1,1326
1333,2211
feature/start start_codon
feature/stop stop_codon
sequence_feature feature/misc
feature/misc sequence_feature
igem#experience
None
 
igem#sampleStatus
Discontinued
igem#status
Deleted
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_I744211/1