Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Peter Nguyen
Date created: 2007-10-23 11:00:00
Date modified: 2015-08-31 04:08:03
LuxN-Tsr Chimeric Receptor D
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_I744213_sequence (Version 1) |
Description
TetR-promoter driven chimeric receptor containing the N-terminal transmembrane sensor domain (1..442) of the quorum-sensing receptor LuxN from Vibrio harveyi fused to the C-terminal signaling domain (216..551) of the E.coli MCP receptor, Tsr. The domains are linked by an NdeI site (translation = 'HM').Notes
The internal NdeI (CATATG) site of the Tsr C-terminal fragment 2 occuring at 772bp of the original protein receptor gene was mutated to (CACATG). This allowed the fusion of the LuxN and Tsr fragments by cloning in an NdeI site upstream at 646bp of Tsr, to test the effects of different fragment lengths.Source
LuxN N-terminal domain is from Vibrio harveyi. Tsr C-terminal domain is from E.coli.| Sequence Annotation | Location | Component / Role(s) |
| Start translation Stop translation LuxN sensory domain fragment 2 Tsr signaling domain fragment 2 point mutation to remove natural NdeI | 1,3 2341,2343 1,1326 1333,2343 1461,1461 | start_codon feature/start feature/stop stop_codon feature/misc sequence_feature sequence_feature feature/misc feature/misc sequence_feature |